pcdna3 sec61b v5 apex (Addgene inc)
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pcdna3 sec61b v5 apex
Pcdna3 Sec61b V5 Apex, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+sec61b+v5+apex/pCDNA3_Sec61B-V5-APEX2+(Plasmid+%2383411)/pm34313336-309-29-31
Average 93 stars, based on 5 article reviews
Pcdna3 Sec61b V5 Apex, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+sec61b+v5+apex/pCDNA3_Sec61B-V5-APEX2+(Plasmid+%2383411)/pm34313336-309-29-31
Average 93 stars, based on 5 article reviews
pcdna3 sec61b v5 apex - by Bioz Stars,
2026-10
93/100 stars
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Sequencing:Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: We also generated a modified pUAST_attB‐V5‐His backbone by assembling a 135bp PCR‐amplified fragment containing the V5 and His tag from pBudCE4.1 (Invitrogen™, V53220) with pUAST‐attB linearized with XbaI. .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST‐mGFP‐Sec61b was produced by assembling pUAST with (i) a 5′ PCR fragment encoding mGFP (amplified from pCR8‐dTormGFP) and (ii) a 3′ PCR fragment encoding mouse Sec61b amplified from Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR-derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST-mGFP-Sec61b was produced by assembling pUAST with (i) a 50 PCR fragment encoding mGFP (amplified from pCR8dTormGFP) and (ii) a 30 PCR fragment encoding mouse Sec61b amplified from Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR‐derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Produced:Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: We also generated a modified pUAST_attB‐V5‐His backbone by assembling a 135bp PCR‐amplified fragment containing the V5 and His tag from pBudCE4.1 (Invitrogen™, V53220) with pUAST‐attB linearized with XbaI. .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST‐mGFP‐Sec61b was produced by assembling pUAST with (i) a 5′ PCR fragment encoding mGFP (amplified from pCR8‐dTormGFP) and (ii) a 3′ PCR fragment encoding mouse Sec61b amplified from Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST-mGFP-Sec61b was produced by assembling pUAST with (i) a 50 PCR fragment encoding mGFP (amplified from pCR8dTormGFP) and (ii) a 30 PCR fragment encoding mouse Sec61b amplified from Polymerase Chain Reaction:Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: We also generated a modified pUAST_attB‐V5‐His backbone by assembling a 135bp PCR‐amplified fragment containing the V5 and His tag from pBudCE4.1 (Invitrogen™, V53220) with pUAST‐attB linearized with XbaI. .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST‐mGFP‐Sec61b was produced by assembling pUAST with (i) a 5′ PCR fragment encoding mGFP (amplified from pCR8‐dTormGFP) and (ii) a 3′ PCR fragment encoding mouse Sec61b amplified from Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR-derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST-mGFP-Sec61b was produced by assembling pUAST with (i) a 50 PCR fragment encoding mGFP (amplified from pCR8dTormGFP) and (ii) a 30 PCR fragment encoding mouse Sec61b amplified from Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR‐derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Amplification:Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: We also generated a modified pUAST_attB‐V5‐His backbone by assembling a 135bp PCR‐amplified fragment containing the V5 and His tag from pBudCE4.1 (Invitrogen™, V53220) with pUAST‐attB linearized with XbaI. .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST‐mGFP‐Sec61b was produced by assembling pUAST with (i) a 5′ PCR fragment encoding mGFP (amplified from pCR8‐dTormGFP) and (ii) a 3′ PCR fragment encoding mouse Sec61b amplified from Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. The coding region of all pUAST plasmids was fully verified by Sanger sequencing before further use. pUAST-mGFP-Sec61b was produced by assembling pUAST with (i) a 50 PCR fragment encoding mGFP (amplified from pCR8dTormGFP) and (ii) a 30 PCR fragment encoding mouse Sec61b amplified from Membrane:Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR-derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR‐derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Generated:Article Title: Torsin and NEP1R1-CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid-dependent and lipid-independent mechanisms. Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR-derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using Article Title: Torsin and NEP1R1‐CTDNEP1 phosphatase affect interphase nuclear pore complex insertion by lipid‐dependent and lipid‐independent mechanisms Article Snippet: .. For dTor membrane domain chimeras, these again used Gibson Assembly to integrate 2 PCR‐derived fragments into pUAST: PCR 1 generated sequence encoding the TMD of Sec61b or dLap1 using |